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Materials and methods MG6 microglia and primary cultured microglia were used in this study. Microglia isolated from young and aged mouse brains by magnetic cell sorting using CD11b-conjugated microbeads were also used. Processings of pro-caspase-1 and pro-IL-1b were analysed by immunoblottings. Secretion of IL-1b was measured by ELISA. The frontal cortex of human brains from AD and no clinical evidence of dementia were used for immunohistochemical analyses.

Differential pathways for the interleukin-1β production activated by chromogranin A and Aβ in microglia