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Background  Concentrated  Gegen Tang  ( GT ) granule is a widely available traditional Chinese medicinal product for the treatment of cold and flu. There was no reliable analytical method available for the quality assessment of  GT  granules.    Methods  An HPLC method with an Agilent Zorbax SB-Phenyl Stablebond column (250 × 4.6 mm, 5 μm) was developed and validated. The mobile phase gradient was a mixture of 0.1% trifluoroacetic acid (TFA) in acetonitrile (ACN) and 0.1% TFA in water. The detection with a diode-array detector was set at 207, 230, 250 and 275 nm. Seven components, namely puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine and pseudoephedrine were selected as marker compounds for the evaluation.    Results  The regression equations revealed good linear relationships (correlation coefficients: 0.9994–0.9998) between the peak areas and concentrations. The recovery was between 98.8% and 101.7% with good precision and accuracy. The quality of  GT  granule from four different manufacturers was evaluated with this newly developed method. Samples from four manufacturers showed similar profiles but contents of the detected markers varied significantly among manufacturers and batches.    Conclusion  A new method using high performance liquid chromatography (HPLC) has been developed for simple and reliable quality control of commercial concentrated  GT  granules. Sensitivity was increased by multi-wavelength detection. The contents of selected marker components in  GT  granules varied significantly among manufacturers and batches, making it necessary to evaluate the quality of concentrated  GT  granules in the market.

Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography