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Methods The various xenogeneic tissues (porcine pericardium, aortic valve, aortic wall and bovine pericardium, aortic valve, aortic wall) were fixed in 0.5% glutaraldehyde solution and implanted into the subcutaneous tissue of the wild type-mouse and the KO mouse for 3 months. The implanted mice were harvested and studied for the histopathology, amounts of calcification and immune responses by serum ELISA.

journal of cardiothoracic surgery

Difference of xenoreactive immune response and patterns of calcification of porcine and bovine tissues in alpha-gal KO and wild type mouse implantation model