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Endothelial injury during the surgical intervention can significantly affect the functional status of the vein. The endothelial layer plays a vital role in the long saphenous vein for ensuring smooth blood flow and the prevention of vasoconstriction and thrombi formation within the blood vessels. There are few histological studies comparing the different vein harvesting techniques that have studied endothelial layer integrity using CD31 on human long saphenous veins.

Validation of the endothelial staining markers CD31 and CD34 in immunohistochemistry of the long saphenous vein.