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Methods 398 AIS girls aged 10 to 18 years, and 367 age-matched healthy girls were recruited. Only patients who had Cobb angles larger than 20° were included in this study. Rs4969198 was selected as tagSNP to cover all of the related polymorphisms on SOCS3. Genotyping was performed using PCR-based Invader assay with the probe sets designed and synthesized by Third Wave. The genotyping results were read with an ABI PRISM7900HT sequence detection system (Applied Biosystems, Foster City, CA). A subgroup of 322 skeletally mature AIS patients who did not received bracing or any other conservative treatment previously were analyzed to define the contribution of rs4969168 on curve severity, body height, body weight and BMI.

Relationship between suppressor of cytokine signaling-3 gene polymorphism and growth pattern of adolescent idiopathic scoliosis