English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Infection by  Mycoplasma hyopneumoniae  and  Actinobacillus pleuropneumoniae , either alone or together, causes serious respiratory diseases in pigs.    Results  To develop an efficient multi-disease subunit vaccine against these pathogens, we produced a chimeric protein called Ap97, which comprises a deletion derivative of the  N -terminal region of the  A. pleuropneumoniae  ApxIII toxin (ApxN) and the R1 and R2 repeats of  M. hyopneumoniae  P97 adhesin (P97C), using an  E. coli  expression system.  The levels of both IgG1 and IgG2a isotypes specific for ApxN and P97C in the sera of Ap97-immunized mice increased, and Ap97 induced the secretion of IL-4 and IFN-γ by mouse splenocytes. Antisera from mice and pigs immunized with Ap97 readily reacted with both native ApxIII and P97 proteins. In addition, immunization with the Ap97 vaccine effectively protected pigs against challenge with both pathogens.    Conclusions  These findings suggest that Ap97 confers immunogenicity, and is an effective vaccine that protects pigs against infection by  M. hyopneumoniae  and  A. pleuropneumoniae .

bmc veterinary research

A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses