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Background  Toxoplasmosis, caused by the obligate intracellular parasite  Toxoplasma gondii , is an important zoonotic disease worldwide. The precise detection of  T. gondii  infection in dogs has important public health significance. In this study, recombinant granule antigen proteins GRA1 and GRA7 were evaluated as potential diagnostic markers for  T. gondii  infection in dogs by an indirect enzyme-linked immunosorbent assay (ELISA).    Results  GRA1 and GRA7 were cloned and expressed in  Escherichia coli , and the recombinant GRA1, GRA7- and  Toxoplasma  lysate antigen (TLA)-based ELISAs were developed and evaluated using the canine positive and negative serum samples for anti- T. gondii  antibodies determined by modified agglutination test (MAT) and indirect fluorescent antibody test (IFAT), showing a seroprevalence of 15.1% by TLA- and GRA1-ELISA, and 15.8% by GRA7-ELISA, and no significant difference was observed ( P >  0.05). When compared with the two reference assays, MAT and IFAT, the GRA7-ELISA showed the highest co-positivity and co-negativity rates. Receiver operating characteristic (ROC) analysis revealed a largest area under curve (AUC) of 0.973 (95% CI, 0.955 to 0.991), and a highest relative sensitivity (93.2%) and specificity (94.0%) for a cut-off value of 0.809 in GRA7-ELISA.    Conclusions  The results of the present study showed that GRA7-ELISA is highly sensitive and specific, and GRA7 is a potential serodiagnostic marker for the detection of  T. gondii  infection in dogs.

Evaluation of recombinant granule antigens GRA1 and GRA7 for serodiagnosis of Toxoplasma gondiiinfection in dogs