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To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and  vice versa . Here we describe an efficient micrografting protocol for  Nicotiana attenuata , a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed  N. attenuata . Our results are consistent with the source-to-sink movement of an sRNA silencing signal.

A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling