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The Seeplex™ TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the  Mycobacterium tuberculosis  complex (MTBC) targets IS 6110  and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.

diagnostic pathology

The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens.