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Aims and methods Glypican 3 (GPC3) belongs to the glypican family of GPI anchored heparan sulfate proteoglycans, which play a crucial role in cellular growth, cell migration and cell differentiation. Several studies have shown GPC3 to be a highly specific marker for hepatocellular carcinoma (HCC) and for differentiating nonand pre-neoplastic liver disease. To systematically investigate the epidemiology of GPC3 expression in non-neoplastic, pre-neoplastic and neoplastic tissues, we used tissue microarray (TMA) technology to analyze the immunohistochemically detectable expression of GPC3 in 3,678 tissue samples from 132 different tumor categories and 31 non-neoplastic and pre-neoplastic tissue types.

Glypican 3 expression in human normal and neoplastic tissue: a tissue microarray analysis on 4338 tissue samples