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Abstract Background The halophilic archaeon <it>Haloferax mediterranei </it>is able to accumulate large amounts of poly(3-hydroxybutyrate-<it>co</it>-3-hydroxyvalerate) (PHBV) with high molar fraction of 3-hydroxyvalerate (3HV) from unrelated carbon sources. A Polyhydroxyalkanoate (PHA) synthase composed of two subunits, PhaCHme and PhaEHme, has been identified in this strain, and shown to account for the PHBV biosynthesis. Results With the aid of the genome sequence of <it>Hfx. mediterranei </it>CGMCC 1.2087, three additional <it>phaC </it>genes (designated <it>phaC1</it>, <it>phaC2</it>, and <it>phaC3</it>) were identified, which encoded putative PhaCs. Like PhaCHme (54.8 kDa), PhaC1 (49.7 kDa) and PhaC3 (62.5 kDa) possessed the conserved motifs of type III PHA synthase, which was not observed in PhaC2 (40.4 kDa). Furthermore, the longer C terminus found in the other three PhaCs was also absent in PhaC2. Reverse transcription PCR (RT-PCR) revealed that, among the four genes, only <it>phaC</it>Hme was transcribed under PHA-accumulating conditions in the wild-type strain. However, heterologous coexpression of <it>phaE</it>Hme with each <it>phaC </it>gene in <it>Haloarcula hispanica </it>PHB-1 showed that all PhaCs, except PhaC2, could lead to PHBV accumulation with various 3HV fractions. The three kinds of copolymers were characterized using gel-permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Their thermal properties changed with the variations in monomer composition as well as the different molecular weights (<it>M</it>w), thus might meet various application requirements. Conclusion We discover three cryptic <it>phaC </it>genes in <it>Hfx. mediterranei</it>, and demonstrate that genetic engineering of these newly identified <it>phaC </it>genes has biotechnological potential for PHBV production with tailor-made material properties.</p

Comparison of four phaC genes from Haloferax mediterranei and their function in different PHBV copolymer biosyntheses in Haloarcula hispanica