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Materials and methods Adult male rats were cannulated into intracerebroventricular (i.c.v). The anticonvulsant effects of rutin were investigated using the pentylenetetrazole (PTZ)-induced seizure model. The animals were placed individually in plastic boxes and observed immediately after PTZ injection for a period of 30 min. Pasipay, diazepam and normal saline were injected i.c.v. at the doses (0.125, 0.25, 0.55,1.1 mg/kg), (10 ml/kg) and (5 mg/kg) respectively 30 minutes before PTZ (90 mg/kg, i.p). The latency to minimal clonic seizure (MCS), generalized tonic-clonic seizure (GTCS) and percent of mortality protection were recorded, as well as the percentages of protection against the mortality.

Intracerebroventricular administration of Pasipay, the hydroalcoholic extract of Passiflora incarnata, suppresses epileptic seizures in rats