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Treated groups One group of mature biofilms was exposed to Penicillin G alone and the other with combination of Penicillin G with Rifampicin for 96 hrs respectively. Again, the discs were retrieved, sonicated and the number of viable bacteria were counted by chemilumiluminesence and culture Results All P.acnes biofilms responded to treatment with a significant reduction in bacterial numbers. Combination therapy was more effective and produced greater reductions (96.6%) of viable bacteria populating the biofilms than penicillin alone, 93.3% (p < 0.01). Complete eradication of the biofilm was not achieved in any cases.

The efficacy of antibiotics against Propionibacterium acnes biofilm infections on spinal implant material