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Background   Tomato yellow leaf curl virus  (TYLCV) is a member of the family  Geminiviridae , genus  Begomovirus . To test the infectivity of TYLCV in tomato plants, an improved protocol for inoculation of  in vitro -cultured tomato plants was developed.    Results  A TYLCV isolate was cloned, sequenced and used to construct a 1.8-mer infectious clone. Three weeks old microshoots of TYLCV-susceptible tomato plants were inoculated with  Agrobacterium tumefaciens  harboring the infectious clone for the TYLCV isolate. After two weeks, the TYLCV symptoms started to appear on the  in vitro -inoculated plants and the symptoms became more severe and pronounced eight weeks post-inoculation. The method was used efficiently to uncover the resistance mechanism against TYLCV in  Solanum habrochaites  accession LA 1777, a wild tomato known for its high resistance to whitefly and TYLCV.    Conclusions  The reported  in vitro -inoculation method can be used to screen tomato genotypes for their responses to TYLCV under controlled conditions and it will be a useful tool for better understanding of the TYLCV biology in tomato plants.

An efficient in vitro-inoculation method for Tomato yellow leaf curl virus