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Background  Bacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.    Methods  We examined the host range, morphology, genome and proteome of the lytic  E. coli  O157 phage rV5, derived from phage V5, which is a member of an  Escherichia coli  O157:H7 phage typing set.    Results  Phage rV5 is a member of the  Myoviridae  family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically isolated with almost 70% of its gene products ORFans. rV5 is closely related to coliphages Delta and vB-EcoM-FY3, and more distantly related to  Salmonella  phages PVP-SE1 and SSE-121,  Cronobacter sakazakii  phage vB_CsaM_GAP31, and coliphages phAPEC8 and phi92. A complete shotgun proteomic analysis was carried out on rV5, extending what had been gleaned from the genomic analyses. Host range studies revealed that rV5 is active against several other  E. coli.

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5