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Methods Indian rhesus macaques were immunized with rBCG-SIV expressing SIV Gag, Env, or Rev-Tat-Nef (RTN) fusion proteins via subcutaneous injection, followed with two boosts with the Gag-, Env-, RT-, or RTN-expressing m8Δ by skin scarification. Eight weeks after the 2nd boost, macaques were challenged up to 5 times with a low dose of SIVmac251 by the rectal route. Cellular and humoral immune responses were analyzed by standard methods. Plasma SIV RNA and cell-associated SIV proviral DNA in peripheral blood and various tissues were monitored by quantitative PCR.

Protection against highly pathogenic SIV by BCG-SIV recombinant priming and attenuated replicating vaccinia-SIV recombinant boosting