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Methods Naive male mauritian cynomolgus macaques were immunized with auxo-GTU-MultiSIV DNA encoding Gag, Nef, Rev and Tat (n=14) or by a combination of auxo-GTUMultiSIV DNA and auxo-GTU-SIV-vifvprvpx (n=12) by the intradermal route followed by electroporation. MHC class I and class II haplotypes were determined by microsatellite analysis. T-cell epitopes were mapped by IFN-g ELISPOT in PBMC by using matrix of 15-mer (overlapping by 11) SIVmac239 Gag, Nef, Rev, Tat, Vif, Vpx and Vpr peptides. Sequence and length of candidate epitopes were further optimized.

Mapping of SIVmac T-cell epitopes in cynomolgus macaques immunized with auxo-GTU-MultiSIV DNA by the intradermal route followed by electroporation