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Methods A V3 specific phage library was constructed from EBV transformed B cells of a clade C HIV-1 infected Indian patient, whose plasma exhibited good neutralization potential against a panel of viruses. Diversity of the constructed phage library was analysed by DNA fingerprinting of 10 randomly selected clones from the unselected library using BstN1 enzyme. One round of biopanning was done against HIV-1 consensus V3C and V3B peptides. scFvs were than characterised for their binding, specifity and expression profile. VH and VL genes of anti-V3 scFvs were sequenced for their preferential gene usage.

Construction of site selected phage library and characterization of anti-V3 scFvs from Indian clade C HIV-1 infected patient