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Methods Peripheral blood mononuclear cells (PBMC) from baboons primed with MVA and boosted with VLPs (n=3) or vaccinated with VLPs only controls (n=2) were stimulated with HIV-1 Gag peptide pools. T cell cytokine production (multiplex TNF-a, IFN-y and IL-2) and memory phenotype was determined by flow cytometry. Human anti-CD28 and CD95 antibodies were used to delineate effector memory (Tem) and central memory (Tcm) T cells.

Induction of HIV-1 Gag-specific memory T cells in Chacma baboons by MVA prime and VLP boost vaccine regimen