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Methods Receptor usage was assessed using CD46 transgenic mouse cells, as well as by CARand CD46-specific mAb blocking studies using human PBMC. Twelve adult rhesus monkeys were inoculated with of 10 viral particles (vp) of replication-competent Ad5 and Ad26 (N=6) or saline (N=6) at weeks -8 and -4, and were vaccinated intramuscularly with 3×10 vp replication-incompetent Ad26-Gag/Pol/Env vectors. At week 2, monkeys were sacrificed to assess immunologic and inflammatory responses at mucosal surfaces.

Adenovirus serotype 26 utilizes CD46 as primary cellular receptor and only transiently activates T lymphocytes following vaccination of rhesus monkeys