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Background After cell entry, HTLV-1 RNA is reverse transcribed and integrated into the host genome using its own reverse transcriptase (RT) and integrase (IN) enzymes. However, some unintegrated DNA circularises into 1 or 2 LTR DNA. Little is known about these unintegrated HTLV-1 DNA circles. Similar to HIV an inhibition of RT should decrease, and an inhibition of IN increase the 1/2 LTR DNA levels.

retrovirology

Circularised 1 and 2 LTR DNA circles are present in freshly- and chronically-infected cell lines and patient PBMCs, indicating ongoing reverse transcriptase usage