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Methods Liposomes containing both a synthetic MPER peptide (662-LELDKWASLWNWFDITNWLWYIK-684) as a peptide antigen, phosphatidylinositol-4-phosphate (PIP) as a lipid antigen, and monophosphoryl lipid A as a potent adjuvant were used as a formulation to immunize mice. MAbs were then produced and tested for binding to MPER, gp41, and PIP and for the ability to neutralize a replication-competent molecular clone of HIV-1 encoding a primary envelope protein and Renilla luciferase as reporter in human peripheral blood mononuclear cells (PBMC).

retrovirology

P05-04. Neutralizing antibodies induced by immunization with liposomal gp41 peptide simultaneously bind to both the 2F5 or 4E10 epitope and lipid epitopes