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Methods Monocyte derived macrophages (MDMs) were isolated from PBMCs, while Monomac-1 cells were obtained from DSMZ. Expression of CD4, HIV-1 co-receptors and FcRs were analysed by FACS staining. Antibody inhibition was determined by p24 release. FcRs involvement was determined using blocking antibodies. Cytokine release was quantified by in house multiplex bead immunoassay. Antibody and immune-complex binding to HIV and FcRs were assessed using an optical SPR Imager. HIV intracellular trafficking was studied by fluorescent microscopy.

P04-33. Mechanisms of HIV-1 antibody mediated inhibition in Monomac-1 and MDM