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Methods Protein G plates were coated with 2F5, 4E10, 2G12, 21.2 (pooled positive HIV-1 human sera), or with anti-IgM antibodies. Primary HIV-1 or pseudoviruses (pv) from clades A [92UG(pv), 93RW(pv)], B [MN, US-1, MN(pv), US-1(pv)], C [GS016], and D [57128(pv)], A07412(pv)] were then added to the plates in the presence or absence of sCD4 to determine the ability of the antibodies to capture virus. The amount of bound virus was determined by a p24 ELISA. The appropriate viruses were also tested in a TZM-bl neutralization assay using mAbs 2F5 and 4E10.

P04-19. Analysis of the binding of multiple clades of HIV-1 by a modified virus capture assay