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Methods Rhesus monkeys were immunized intramuscularly using a plasmid DNA prime/recombinant Ad5 boost regimen. Experimental animals received vectors expressing genes encoding SIVmac239 env and gag/pol, and control animals received empty vectors. Monkeys were challenged 4 months after their final immunization with a series of 12 weekly intra-rectal exposures of SIVsmE660 in one study, and SIVmac251 in a second study. The challenge dose for each virus was titered to achieve ~50% infection per-inoculation for control animals. Time to acquisition of infection was monitored by weekly assessment of plasma SIV RNA levels.

P19-57 LB. Gene-based vaccination protects against mucosal infection by a heterologous highly pathogenic SIV isolate in rhesus monkeys