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Background Recombinant MVA expressing HIV genes are currently being tested in phase I and II vaccine trials. During efforts to make rMVA/HIV candidate vaccines, we found variable stability of inserted gene expression. Maintaining stability upon repeated passage for vaccine production while keeping high expression of inserted gene for increased immunogenicity is highly desirable. In continuation of efforts to improve stability, we now compare three insertion sites in the MVA genome using the same HIV env gene.

retrovirology

P19-52 LB. Assessing and restructuring foreign gene insertion sites for enhanced stability of modified Vaccinia virus ankara recombinants expressing HIV genes