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Methods T-cell subsets were sorted from HIV-infected and -uninfected individuals by polychromatic flow cytometry. Expression of lineage-specific transcription factors and cytokines was quantified by real-time RT-PCR and ELISA, respectively. HIV replication and integration were measured by HIV-p24 ELISA and real-time PCR, respectively. The frequency of T-cell subsets was analyzed in HIVinfected and -uninfected individuals.

retrovirology

P16-54 LB. Blood CCR6+ Th17 and Th1Th17 but not CCR6neg Th1 cells are targets for HIV replication and their frequency is diminished in HIV-infected subjects