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Methods Ad5hr-SIVenv/rev, -SIVgag, and -GFP recombinants were given to rhesus macaques by the IN/IT, sublingual, rectal or vaginal routes at weeks 0 and 12. Controls received Ad5hr-GFP only. Blood, bronchial alveolar lavage (BAL), and rectal biopsies were collected one week after each Ad immunization (weeks 1 and 13) and examined for GFP expression among lymphocytes (CD3, CD4, CD8, B, NK), macrophages, dendritic and epithelial cells by flow cytometry. Two weeks post each Ad (weeks 2 and 14), select macaques were sacrificed and multiple tissues fixed for later histochemical staining. Flow analyses were also expanded at necropsy to include jejunum, tonsils and lymph nodes.

retrovirology

P19-35. Replicating adenovirus type 5 host range recombinant vaccine biodistribution in rhesus macaques following multiple mucosal routes of immunization