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Methods Virus-specific CD8 T-cell responses (n = 74) including CMV, EBV, Flu and HIV-1 were characterized using tetramer complexes or peptide stimulation and analyzed for proliferation capacity, expression of CD127 and perforin. CCR7, CD45RA, PD-1 and CD57 were used to assess T-cell differentiation/exhaustion. Simultaneous expression of IFNγ, IL-2, TNFα and perforin was analysed by polychromatic flow cytometry.

P16-32. Antigen exposure regulates the balance between proliferating and cytotoxic subsets of virus-specific CD8 T-cells