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Methods We used platelets sampled from healthy, HIV-1,2 negative, normal blood donors. Platelet-Rich Plasma (PRP) was incubated after addition thrombin receptor agonist peptide or HIV env glycoprotein. PRP were centrifuged and platelet free supernatant was stored. Two recombinant gp120MN (produced in insect cells using the baculovirus expression system) and synthetic peptides of gp41MN, and mAbs to gp41: D50 and 2F5, were all obtained through the AIDS Research and Reference Reagent Program. Soluble CD62p and RANTES production in cultures were measured by specific ELISA.

P10-09. Altered release of RANTES from human, normal platelets: contribution of distinct HIV-1MN gp41 peptides