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Methods We have studied the effects of human milk (HM) and seminal plasma (SP) on interfering with the capture of HIV-1 through binding to DC-SIGN on dendritic cells and prevent the subsequent transfer of HIV-1 to CD4 cells. HM and SP were tested in Raji-DC-SIGN and immature dendritic cell transfer assays to determine interference with HIV-1 capture and transfer. Fractionation and MALDI-TOF biochemical analysis was utilized to identify proteins with DC-SIGN binding activity.

P07-10. Natural compounds in bodily fluids which bind DC-SIGN and prevent HIV-1 capture and transfer to CD4 cells