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Materials and methods The process through which HIV-1 is endocytosed was studied using different reagents (e.g. chlorpromazine, cholera toxin B, water-soluble cholesterol, colchicine, cytochalasin B, filipin, jasplakinolide, methyl-beta-cyclodextrin, paclitaxel, and vinblastine) and experimental strategies (e.g. transfection of JAR cells with various expression vectors, virus internalization test, infection assay, confocal laser scanning, co-localization analysis and digital image preparation).

HIV-1 internalization in polarized human trophoblasts occurs through a peculiar endocytic pathway