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Methods Phage displayed peptide libraries were screened with PSIRNA structures. The Trp-rich peptide motifs were optimized for specific binding on spot synthesis peptide membranes. The best binding peptide was expressed intracellularly in fusion with RFP or linked to a protein transduction domain (PTD) for intracellular delivery. The effects on virion production were analyzed using pseudotyped lentiviral particles.

Optimization and antiviral analysis of peptide ligands for the HIV-1 packaging signal PSI