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Background To study the meaning of HIV indeterminate serology (HIVi), evaluating the deferral criteria of HIV screening test, we looked for donors (D) whose blood donations (BD) were discarded for HIVi from 1/1/1999 till 31/12/ 2005. The test system of blood donations is Prism Abbott Anti-HIV 1/2; HIV supplemental test was New Lav-Blot I/ II-Bio-Rad till 2002 and Inno-LiaTM HIV I/II-Innogenetics afterwards. We perform a NAT test in all single donation since 2000, detecting HIV-1 and HCV RNA; since 2/2004 we perform Procleix® UltrioTM Assay. Grey zone (GZ) for HIV screening test means 20% inferior to the cutoff (ICO). D that have been deferred for HIV serology but didn't obey to these criteria were excluded.

HIV indeterminate serology in a Portuguese blood donor population – review of seven years