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Materials and methods Patients with perinatal HIV infection (n = 19, ages 11–18 yr, on HAART) were classified as immunologic responders (IR+; CD4>25%), and virologic responders (VR; plasma HIV RNA < 400 copies/mL). mDC (Lin-, HLA DR+CD11c+) and pDC (Lin-, HLA DR+ CD11c-) were evaluated in a novel whole blood assay by flow cytometry for expression of maturation markers CD83, CD80, homing receptor CCR7 and intracellular cytokines (TNF-α and IFN-α) after short-term stimulation with a TLR7/8 agonist, resiquimod.

Impaired CCR7 Expression on Plasmacytoid Dendritic Cells in HIV Infected Children on HAART With Virologic Failure