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Materials and methods Peripheral blood was obtained from 5 HIV patients receiving HAART for 2–12 years. Three patients had 400<HIV RNA copies/ml with between 529 and 1,588 CD4 T cells/ μl. Other two had 915 and 453,000 RNA copies/ml with 235 and 266 CD4 T cells/μl. Each T cell subset was sorted by FACSAria, washed and DNA was isolated. Proviral HIV env C2-V3 genes were PCR amplified, cloned and sequenced, and were phylogenetically analyzed by using MEGA (v.2.1).

Characterization of Proviral HIV Latency in Different T Cell Subsets of Patients Undergoing HAART