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In the present investigation the fungi,  Aspergillus niger  and  Nigrospora  sp. were employed for decolorization of Synozol red HF-6BN. Decolorization study showed that  Aspergillus niger  and  Nigrospora  sp. were able to decolorize 88% and 96% Synozol red 6BN, respectively, in 24 days. It was also studied that 86% and 90% Synozol red containing of dye effluent was decolorized by  Aspergillus niger  and  Nigrospora  sp. after 28 days of incubation at room temperature. A fungal-based protein with relative molecular mass of 70 kDa was partially purified and examined for enzymatic characteristics. The enzyme exhibited highest activity at temperature ranging from 40-50°C and at pH=6.0. The enzyme activity was enhanced in the presence of metal cations. High performance liquid chromatography analysis confirmed that these fungal strains are capable to degrade Synozol red dye into metabolites. No zones of inhibition on agar plates and growth of  Vigna radiata  in the presence of dye extracted sample, indicated that the fungal degraded dye metabolites are nontoxic to beneficial micro-flora and plant growth.  Aspergillus niger  and  Nigrospora  sp. have promising potential in color removal from textile wastewater-containing azo dyes.

Decolorization and detoxification of Synozol red HF-6BN azo dye, by Aspergillus niger and Nigrosporasp