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Methods Screening for obesity using CDC 2000 criteria was done in 2121 female adolescents aged 13-14 years old in Yogyakarta. BMI > 95 percentile was considered as obese. Among the obese subjects, 78 agreed to be enrolled for this study. HOMA-IR > 3.16 was used to determine the insulin resistance status. DNA was isolated from peripheral blood and UCP2 -866 G/A, UCP3 -55C/T, GHSR1a rs2922126, GHSR1a rs509035 and Pro12Ala PPARg2 genotypes were analyzed by PCRRFLP. UCP2 45bp ins/del genotype was analyzed by PCR.

international journal of pediatric endocrinology/international journal of pediatric endocrinology

Association between insulin resistance with UCP2 -866G/A, UCP2 45BP INS/DEL, UCP3 -55C/T, GHSR1A RS2922126, GHSR1A RS509035 and PRO12ALA PPARΓ2 gene polymorphisms in obese female adolescents in Yogyakarta, Indonesia