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Methods 30 fasted, recreationally trained males (22.5 yrs; 83.1 kg; 178.4 cm) consumed 120 mg/kg/bw of BCAA, 60 mg/kg/ bw of leucine, or a placebo. The supplements were consumed in three equal doses at 30 minutes before RE, immediately prior to RE, and immediately post RE. The participants completed 4 sets of both leg press and knee extension at 80% of their 1 RM to failure (~8–12 reps). Rest periods of 2.5 minutes were given between both sets and exercises. Percutaneous muscle biopsies of the vastus lateralis were obtained at: baseline, and 30 minutes, 2 hours, and 6 hours post RE. The phosphorylated states of both mTOR and 4E-BP1 were assessed through the use of an ELISA with a primary antibody specific to phosphorylated mTOR [pS2448] and a phosphoELISA kit for phosphorylated 4E-BP1 [pT46], respectively. Other serum and muscle variables were analyzed as part of a greater, overall study, but only the phosphorylated mTOR and 4E-BP1 are reported in this abstract. Delta values of mTOR and 4EBP1 were analyzed using a 3 (group) × 4 (time) repeated measures MANOVA. Separate ANOVAs for each criterion variable were utilized as follow-up tests. Significant main effects were determined Bonferroni post-hoc tests. Significant interactions discovered in the ANOVAs were assessed by independent samples T-tests. SPSS version 15.0 was utilized throughout this analysis.

journal of the international society of sports nutrition

The effects of oral BCAAs and leucine supplementation combined with an acute lower-body resistance exercise on mTOR and 4E-BP1 activation in humans: preliminary findings