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Materials and methods The single nucleotide polymorphism (SNP) at position 543 of exon 5 of FOXP3 was investigated in 58 caucasian children patients with KS (F:30, M:28), recruited at our Paediatric Reumatology Unit. In 6 patients we could evaluate the percentage of circulating CD4+ T cells co-expressing CD25 and FOXP3 by flow cytometry, mRNA transcripts of FOXP3 in PBMCs by Real Time PCR (with Rnase P as internal control gene), and the levels of IL-17 in PBMCs supernatants (5 × 104 cells) by a quantitative immunoassay Kit.

FOXP3 Polymorphism and gene expression in Italian patients with Kawasaki Syndrome