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Background 13C Dynamic Nuclear Polarization (DNP) with rapid dissolution together with Magnetic Resonance Chemical Shift Imaging (CSI) have been used for non-invasive real-time metabolic assessment in cardiac experimental models on a clinical 3T scanner. Here, we report an in vivo comparison of hyperpolarized [1-13C] pyruvate and [1-13C] acetate perfusion and metabolism: a method based on a 3D Spiral CSI sequence is presented for obtaining spatially and spectrally-resolved information on whole heart cardiac metabolism.

3D cardiac Chemical Shift Imaging of [1-13C] hyperpolarized acetate and pyruvate in pigs