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Summary This study describes a novel T1-weighted MR molecular imaging approach for sparse epitopes, such as the avb3integrin receptor in atherosclerotic angiogenesis, utilizing a soft Mn-based nanoparticle with high-relaxivity augmented by minute amounts of surface gadolinium. This new agent utilizes about 1/30th the amount of lanthanide used for PFC paramagnetic particles and reduces gadolinium exposure by 300-fold compared with clinical single dose Gd-DTPA. Background We have previously reported in hyperlipidemic rabbit models that integrin-targeted gadolinium perfluorocarbon nanoparticles can effectively assess plaque angiogenesis using MRI. However, the concern for Nephrogenic Systemic Fibrosis (NSF) has motivated the development of alternative technologies that dramatically lower Gd use. We have developed a manganese oleate based nanoparticle with high longitudinal relaxivity for fibrin-specific imaging of thrombus. However, for sparse receptors, such as neovascular integrins, preliminary studies revealed that paramagnetic nanoparticles with greater T1w effects were needed. The objective of this research was to evaluate a manganese-gadolinium nanocolloid (MnGd NC) with gadolinium levels of less than 300X current clinical dosage levels, while providing high levels of T1-weighted MR imaging of sparse angiogenic avb3-integrin expression in vivo. Methods A new nanocolloid comprised of a bivalent manganese oleate (0.49±0.02 mg Mn/ml) /polysorbate core encapsulated with phospholipid surfactant enriched with 1.25 mole% Gd-DOTA-cholesterol and 0.3 mole% of quinolone-derived peptidomimetic avb3-integrin antagonistcoupled to phosphatidylethanolamine through a PEG (2000) spacer was produced (Diam., 134±2 nm.; Zeta, -25±02mv; PDI, 0.13±0.03). Hyperlipidemic New Zealand White rabbits (n=8), fed a 0.25% cholesterol diet (egg-derived) for 12 months, received avb3-integrinMnGd NC or nontargeted MnGd NC; avb3-IntegrinMnGd NC was also administered to rabbits fed a normal chow (n=4). Dynamic 3T MR imaging of the descending thoracic aorta was performed over 2 hours post-injection. Molecular imaging results were corroborated microscopically using fluoresence microscopy. Results MR signal enhancement at 2 hours was increased 18.7 ±1.95% when averaged over all slices and voxels of the aortic wall in hyperlipidemic rabbits treated with avb3integrin-MnGd NC. (Fig) Signal enhancement due to nonspecific neovascular accumulation of nontargeted MnGd NC was significantly less, (2.5±1.17%, p<0.05). Rabbits fed a normal diet and treated with avb3-integrin-MnGd NC had the signal increase, 3.17±1.94%, which did not differ from the nontargeted control. The MRI pattern observed was spatially heterogeneous along both transverse and longitudinal planes of the descending aorta, and predominantly localized between the left subclavian and the diaphragm. Immunohistochemistry corroborated the prominent, neointimal proliferation among cholesterol-fed, atherosclerotic rabbits and the

Probing atherosclerotic angiogenesis with new manganese-based nanocolloid for T1-weighted MRI