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Methods MI was induced in 5 minipigs by a 120-min occlusion of the LAD followed by reperfusion. PET and 3T-MRI studies were performed on the same day 1 or 3 days post MI. Microspheres were injected directly after the MR perfusion acquisition. First pass contrast enhanced myocardial MR images were obtained at rest after a 0.05 mmol/kg BW Gdinjection with a FLASH sequence on a 3 Tesla magnet (TIM Trio, Siemens Medical Solutions). Myocardial perfusion (ml/min/g) was quantified from arterial input and myocardial output function by using a Patlak plot analysis. From dynamic 13N-ammonia PET studies, MBF was quantified by fitting to a validated 3-compartment model. Quantitative MBF measurements from 3T-MRI were compared to that of PET using a modified 17 segment AHA model. A total of 64 segments were evaluated. Post mortem the pig hearts were harvested and cut into 8 mm slices according to the short axis MRI prescription. Eight sectors per slice were used to compare matching microsphere flow measurements and MRI values.

journal of cardiovascular magnetic resonance

2098 Magnetic resonance imaging at 3 Tesla to quantify regional myocardial blood flow after myocardial infarction: comparison with 13N-ammonia positron emission tomography and microspheres