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Materials and methods In the present work, we evaluated the in vivo melanomatargeting ability of a nanovector (HFt-MSH-PEG) based on human protein ferritin (HFt), functionalized with both melanoma-targeting melanoma stimulating hormone (a-MSH) and stabilizing poly(ethylene glycol) (PEG) molecules. We used two independent and complementary techniques, such as whole-specimen confocal microscopy and magnetic resonance imaging, to detect the in vivo localization of NP constructs endowed with suitable tracers (i.e., fluorophores or magnetic metals).

In vivo targeting of cutaneous melanoma using an MSH-engineered human protein cage bearing fluorophore and MRI tracers