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Abstract Fractionation and characterization of gonadotropins (GtH) from <it>Fundulus heteroclitus </it>pituitary extracts were carried out using a biocompatible liquid chromatographic procedure (Pharmacia FPLC system). Chromatographic fractions were monitored for gonadotropic activities (induction of oocyte maturation and steroid production) using homologous follicle bioassays <it>in vitro</it>. Size-exclusion chromatography eluted gonadotropic activity in one major protein peak (<it>M</it>r ~ 30,000). Anion-exchange and hydrophobic-interaction chromatography (HIC) yielded two distinct peaks of 17beta-estradiol (E2)- and 17alpha-hydroxy,20beta-dihydroprogesterone (DHP)-promoting activity with associated oocyte maturation. Two-dimensional chromatography (chromatofocusing followed by HIC) resolved pituitary extracts into two active fractions; both induced E2 synthesis, but one was relatively poor in eliciting DHP and testosterone production. Thus, using homologous bioassays, at least two quantitatively different gonadotropic (steroidogenic) activities: an E2-promoting gonadotropin (GtH I-like) and a DHP-promoting gonadotropin (GtH II-like), which has a lower isoelectric point but greater hydrophobicity than the former, can be distinguished from <it>F. heteroclitus </it>pituitaries by a variety of chromatographic procedures. This study complements previous biochemical and molecular data in <it>F. heteroclitus </it>and substantiates the duality of GtH function in a multiple-spawning teleost.</p

reproductive biology and endocrinology

Fundulus heteroclitus gonadotropins.5: Small scale chromatographic fractionation of pituitary extracts into components with different steroidogenic activities using homologous bioassays.