English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  RNA binding motif 5 ( RBM5 ) is a tumor suppressor gene that modulates apoptosis through the regulation of alternative splicing of apoptosis-related genes. Our previous studies suggested that  RBM5  expression was negatively correlated with the expression of epidermal growth factor receptor ( EGFR ) in non-small cell lung cancer (NSCLC) tissues. This study was aimed at determining whether  RBM5  is able to regulate  EGFR  expression.    Methods  Both  in vitro  and  in vivo  studies were carried out to determine the effect of  RBM5  on the expression of  EGFR . Lentiviral vector-mediated  RBM5  overexpression was employed in lung adenocarcinoma cell line A549. A549 xenograft mice were treated with recombinant  RBM5  plasmid carried by attenuated  Salmonella  typhi Ty21a. Real-time quantitative polymerase chain reaction and Western blot were carried out to detect  RBM5  and  EGFR  expression.    Results  Both  in vivo  and  in vitro  studies indicated that the expression of  EGFR  mRNA and protein was decreased significantly in the  RBM5  overexpression group compared to control groups as shown by real-time PCR and Western blot analysis. We identified that  RBM5  overexpression inhibited  EGFR  expression both in A549 cells and in A549 xenograft mice model.    Conclusions  Our study demonstrated that  EGFR  expression is regulated by  RBM5  in lung adenocarcinomas cells either in a direct or indirect way, which might be meaningful with regards to target therapy in lung cancer.

Lentiviral vector-mediated RBM5 overexpression downregulates EGFR expression in human non-small cell lung cancer cells