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Materials and methods Male BALB/c mice were sensitized to purified house dust mite extract (HDM; 25 μg) without exogenous adjuvant 5 days/week for 5 weeks (day 0-32). On day 21 designated groups were treated with fluticasone propionate (F; 10ug/animal, BID) until the end of the study. On day 35, designated groups were instilled with influenza (IFZ; A/HKx31[H3N2]; 10 PFU). On day 39 (4 days pi) and 49 (14 days pi), groups were evaluated for airway hyperreactivity (AHR), lung inflammation (BAL), viral titer, and histopathology.

Viral exacerbation of chronic airway inflammation in BALB/c mice sensitized with house dust mite (HDM): a role for corticosteroids?