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Background  Investigations of  Plasmodium vivax  are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in  in vitro  cultures. Contamination of  P. vivax  isolates with host leukocytes and platelets is detrimental to a range of  ex vivo  and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of  P. vivax  IRBCs by CF11 cellulose filtration.    Methods and Results  Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 10 3  per μl [95%CI 5.2–13.5] to 0.01 × 10 3  [95%CI 0.01–0.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 10 3  per μl [95%CI 107.5–315.7] to 0.8 × 10 3  per μl [95%CI -0.7–2.2]. Analysis of 30  P. vivax  blood samples before and after CF11 filtration showed only a minor loss in parasitaemia (≤ 7.1% of initial counts). Stage specific retention of  P. vivax  IRBCs was not observed.    Conclusion  CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free  P. vivax -infected erythrocytes from field isolates.

Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood