English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The knowledge on population structure of the parasite isolates has contributed greatly to understanding the dynamics of the disease transmission for designing and evaluating malaria vaccines as well as for drug applications.  msp-1  and  msp-3α  genes have been used as a genetic marker in population studies of  Plasmodium vivax  isolates. In this study,  msp-3α  was compared and assessed with  msp-1  marker in order to find whether  msp-3α  is a reliable genetic marker for  P. vivax  population studies.    Methods  This comparative study was designed and carried out as the first assessment of diversity in  Pvmsp-3α  gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the 50 northern and 94 southern  P. vivax  isolates from Iran, which had been analysed before for  msp-1  gene.    Results  Three allele size as, Type A (1.8 kb), Type B (1.5 kb) and Type C (1.2 kb) have been detected among both northern and southern isolates based on PCR results. Type C (70%) and Type A (68.7%) were the predominant fragments among northern and southern parasites, respectively. 99 distinct  Pvmsp -3α fragments defined by the size were detected in the 94 southern samples by PCR analysis. However, no mixed genotype infections have been detected among northern isolates. Based on restriction pattern from digestion with  Hha I  and  Alu I  12 and 49 distinct allelic variants have been detected among 50 northern and 94 southern isolates. However, based  on msp-1  gene, 30 distinct variants identified in all 146-sequenced Iranian  P. vivax  isolate.    Conclusion  The results suggested that PCR-RFLP on  msp-3α  gene is an adequate, applicable and easily used technique for molecular epidemiology studies of  P. vivax  isolates without the need for further sequencing analysis.

Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax