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Materials and methods During April 2011-November 2012, 1564 patients aged >5 years were included in a larger fever study from seven secondary hospitals in India located in Assam (Tezpur), Bihar (Raxaul), Chhattisgarh (Mungeli), Maharashtra (Ratnagiri), Andhra Pradesh (Anantapur) and Tamil Nadu (Oddanchatram and Ambur). Routine microscopy, genus-specific mitochondrial PCR and the rapid immunochromatographic test (RDT) Parahit TotalTM (Span Diagnostics Ltd, Surat, India) were performed. Species-specific 18S PCR or sequencing was performed on genus PCR positive samples. Samples with discordancy between PCR and RDT were retested by PCR from the extraction step. PCR was considered as gold standard.

Malaria prevalence among patients with acute undifferentiated fever in secondary hospitals in India